Article
Structure of a stabilizing disulfide bridge mutant that closes the active-site cleft of T4 lysozyme.
Protein science : a publication of the Protein Society - 1 Jan 1992
Jacobson R H, Matsumura M, Faber H R, Matthews B W
Abstract excerpt
The engineered disulfide bridge between residues 21 and 142 of phage T4 lysozyme spans the active-site cleft and can be used as a switch to control the activity of the enzyme (Matsumura, M. & Matthews, B.W., 1989, Science 243, 792-794). In the oxidized form the disulfide increases the melting tem...
Topics
- Bacteriophage T4
- Cysteine
- Disulfides
- Enzyme Stability
- Models, Molecular
- Muramidase
- Mutation
- Oxidation-Reduction
- Protein Conformation
- Protein Engineering
- X-Ray Diffraction