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Article

Structure of a stabilizing disulfide bridge mutant that closes the active-site cleft of T4 lysozyme.

Protein science : a publication of the Protein Society - 1 Jan 1992

Jacobson R H, Matsumura M, Faber H R, Matthews B W

Abstract excerpt

The engineered disulfide bridge between residues 21 and 142 of phage T4 lysozyme spans the active-site cleft and can be used as a switch to control the activity of the enzyme (Matsumura, M. & Matthews, B.W., 1989, Science 243, 792-794). In the oxidized form the disulfide increases the melting temperature of the protein by 11 degrees C at pH 2. The crystal structure of this mutant lysozyme has been determined in...

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Identifiers

PMID: 1304882
DOI: 10.1002/pro.5560010106
PMCID: PMC2142079

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Source receipt: pubmed, retrieved 7 Sept 2026.

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