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Article

Evaluation of peptide-aldehyde inhibitors using R188I mutant of SARS 3CL protease as a proteolysis-resistant mutant.

Bioorganic & medicinal chemistry - 1 Nov 2008

Akaji Kenichi, Konno Hiroyuki, Onozuka Mari, Makino Ayumi, Saito Hiroyuki, Nosaka Kazuto

Abstract excerpt

The 3C-like (3CL) protease of the severe acute respiratory syndrome (SARS) coronavirus is a key enzyme for the virus maturation. We found for the first time that the mature SARS 3CL protease is subject to degradation at 188Arg/189Gln. Replacing Arg with Ile at position 188 rendered the protease resistant to proteolysis. The R188I mutant digested a conserved undecapeptide substrate with a K(m) of 33.8 microM and...

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Identifiers

PMID: 18845442
DOI: 10.1016/j.bmc.2008.09.057
PMCID: PMC7126698

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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