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Article

Importance of the His-298 residue in the catalytic mechanism of the Streptomyces R61 extracellular DD-peptidase.

The Biochemical journal - 1 Mar 1992

Hadonou A M, Jamin M, Adam M, Joris B, Dusart J, Ghuysen J M, Frère J M

Abstract excerpt

Among the active-site-serine penicillin-recognizing proteins, the Streptomyces R61 extracellular DD-peptidase is the only one to have a His-Thr-Gly sequence [instead of Lys-Thr(Ser)-Gly] in 'box' VII. The His residue was replaced by Gln or Lys. Both mutations induced a marked decrease in the rates of both tripeptide substrate hydrolysis and acylation by benzylpenicillin and cephalosporin C. The rate of hydrolysis...

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Identifiers

PMID: 1546964
DOI: 10.1042/bj2820495
PMCID: PMC1130807

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Source receipt: pubmed, retrieved 7 Sept 2026.

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