Article
Evidence from a mutant beta-lactamase for the mechanism of beta-lactamase-catalysed depsipeptide aminolysis.
The Biochemical journal - 15 Mar 1991
Mazzella L J, Pazhanisamy S, Pratt R F
Abstract excerpt
The Ser-70----Gly mutant of the TEM-1 beta-lactamase, where the active-site serine hydroxy group has been lost, does not catalyse the hydrolysis of either benzylpenicillin or N-(phenylacetyl)glycyl depsipeptides. This is as would be expected for a double-displacement mechanism where the Ser-70 becomes acylated at an intermediate stage. Further, however, the mutant enzyme, unlike the wild-type, does not catalyse...
Topics
- Amines
- Catalysis
- Circular Dichroism
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Gene Expression Regulation, Enzymologic
- Hydrolysis
- Mutation
- Peptides
- Plasmids
- Spectrophotometry, Ultraviolet