A new kinetic mechanism for the concomitant hydrolysis and transfer reactions catalyzed by bacterial DD-peptidases.

doi:10.1021/bi00079a026

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Article

A new kinetic mechanism for the concomitant hydrolysis and transfer reactions catalyzed by bacterial DD-peptidases.

Biochemistry - 20 Jul 1993

Jamin M, Wilkin J M, Frère J M

Abstract excerpt

In the presence of an adequate nucleophilic acceptor substrate (A) and ester and thiolester donor substrates (S), the Streptomyces R61 soluble DD-peptidase catalyzes both hydrolysis and acyl group transfer reactions. Simple bisubstrate models do not explain the variation of the transfer to hydrol...

Topics

Acylation
Asparagine
Catalysis
Hydrolysis
Kinetics
Muramoylpentapeptide Carboxypeptidase
Mutagenesis, Site-Directed
Mutation
Serine
Streptomyces

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