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Article

Change in substrate preference of Streptomyces aminopeptidase through modification of the environment around the substrate binding site.

Applied and environmental microbiology - 1 Dec 2006

Arima Jiro, Uesugi Yoshiko, Iwabuchi Masaki, Hatanaka Tadashi

Abstract excerpt

We attempted to alter the substrate preference of aminopeptidase from Streptomyces septatus TH-2 (SSAP). Because Asp198 and Phe221 of SSAP are located in the substrate binding site, we screened 2,000 mutant enzymes with D198X/F221X mutations. By carrying out this examination, we obtained two enzymes; one specifically hydrolyzed an arginyl derivative, and the other specifically hydrolyzed a cystinyl derivative...

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Identifiers

PMID: 17028223
DOI: 10.1128/AEM.01460-06
PMCID: PMC1694256

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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