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Article

Mutational analysis of a key residue in the substrate specificity of a cephalosporin acylase.

Chembiochem : a European journal of chemical biology - 7 Jun 2004

Otten Linda G, Sio Charles F, van der Sloot Almer M, Cool Robbert H, Quax Wim J

Abstract excerpt

beta-Lactam acylases are crucial for the synthesis of semisynthetic cephalosporins and penicillins. Unfortunately, there are no cephalosporin acylases known that can efficiently hydrolyse the amino-adipic side chain of Cephalosporin C. In a previous directed evolution experiment, residue Asn266 of the glutaryl acylase from Pseudomonas SY-77 was identified as being important for substrate specificity. In order to...

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Identifiers

PMID: 15174165
DOI: 10.1002/cbic.200300764

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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