Article
Point mutations of two arginine residues in the Streptomyces R61 DD-peptidase.
The Biochemical journal - 1 Apr 1992
Bourguignon-Bellefroid C, Joris B, Van Beeumen J, Ghuysen J M, Frère J M
Abstract excerpt
Incubation of the exocellular DD-carboxypeptidase/transpeptidase of Streptomyces R61 with phenylglyoxal resulted in a time-dependent decrease in the enzyme activity. This inactivation was demonstrated to be due to modification of the Arg-99 side chain. In consequence, the role of that residue was...
Topics
- Amino Acid Sequence
- Arginine
- Bacterial Proteins
- Base Sequence
- Enzyme Activation
- Leucine
- Molecular Sequence Data
- Muramoylpentapeptide Carboxypeptidase
- Mutagenesis, Site-Directed
- Mutation
- Penicillins
- Phenylglyoxal
- Protein Binding
- Serine
- Streptomyces