Article
A genome annotation-driven approach to cloning the human ORFeome.
Genome biology - 1 Jan 2004
Collins John E, Wright Charmain L, Edwards Carol A, Davis Matthew P, Grinham James A, Cole Charlotte G, Goward Melanie E, Aguado Begoña, Mallya Meera, Mokrab Younes, Huckle Elizabeth J, Beare David M, Dunham Ian
Abstract excerpt
We have developed a systematic approach to generating cDNA clones containing full-length open reading frames (ORFs), exploiting knowledge of gene structure from genomic sequence. Each ORF was amplified by PCR from a pool of primary cDNAs, cloned and confirmed by sequencing. We obtained clones representing 70% of genes on human chromosome 22, whereas searching available cDNA clone collections found at best 48%...
Topics
- Chromosomes, Human, Pair 22
- Cloning, Molecular
- Computational Biology
- DNA, Complementary
- Databases, Genetic
- Genome, Human
- Genomics
- Humans
- Open Reading Frames
- Polymerase Chain Reaction
- Polymorphism, Single Nucleotide
- Proteome