A biomedically enriched collection of 7000 human ORF clones.

doi:10.1371/journal.pone.0001528

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Article

A biomedically enriched collection of 7000 human ORF clones.

PloS one - 30 Jan 2008

Rolfs Andreas, Hu Yanhui, Ebert Lars, Hoffmann Dietmar, Zuo Dongmei, Ramachandran Niro, Raphael Jacob, Kelley Fontina, McCarron Seamus, Jepson Daniel A, Shen Binghua, Baqui Munira M A, Pearlberg Joseph, Taycher Elena, DeLoughery Craig, Hoerlein Andreas, Korn Bernhard, LaBaer Joshua

Abstract excerpt

We report the production and availability of over 7000 fully sequence verified plasmid ORF clones representing over 3400 unique human genes. These ORF clones were derived using the human MGC collection as template and were produced in two formats: with and without stop codons. Thus, this collection supports the production of either native protein or proteins with fusion tags added to either or both ends. The...

Topics

Cloning, Molecular
Genetic Predisposition to Disease
Humans
Open Reading Frames
Sequence Analysis, DNA

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Identifiers

PMID: 18231609
DOI: 10.1371/journal.pone.0001528
PMCID: PMC2211400

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