Article
Detection of multiallele polymorphisms within gene sequences by GC-clamped denaturing gradient gel electrophoresis.
American journal of human genetics - 1 Mar 1992
Sheffield V C, Beck J S, Nichols B, Cousineau A, Lidral A C, Stone E M
Abstract excerpt
The ability to efficiently detect DNA polymorphisms is essential for the completion of a high-resolution polymorphic linkage map of the human genome. Currently the most informative polymorphisms are the multiallelic dinucleotide repeat polymorphisms. However, many gene sequences lack an associated dinucleotide repeat sequence. We used GC-clamped denaturing gradient gel electrophoresis to screen for DNA...
Topics
- Alleles
- Antigens
- Arrestin
- Base Sequence
- Chromosome Mapping
- DNA
- Electrophoresis, Gel, Pulsed-Field
- Eye Proteins
- Genes, Tumor Suppressor
- Genes, p53
- Genetic Linkage