Article
Validation of double gradient denaturing gradient gel electrophoresis through multigenic retrospective analysis.
Clinical chemistry - 1 Jan 1999
Cremonesi L, Carrera P, Fumagalli A, Lucchiari S, Cardillo E, Ferrari M, Righetti S C, Zunino F, Righetti P G, Gelfi C
Abstract excerpt
Among established techniques for the identification of either known or new mutations, denaturing gradient gel electrophoresis (DGGE) is one of the most effective. However, conventional DGGE is affected by major drawbacks that limit its routine application: the different denaturant gradient ranges...
Topics
- Cystic Fibrosis Transmembrane Conductance Regulator
- DNA
- DNA Mutational Analysis
- Electrophoresis
- Globins
- Humans
- Mutation
- Nucleic Acid Denaturation
- Polymorphism, Genetic
- Reproducibility of Results
- Tumor Suppressor Protein p53