Comprehensive detection of single base changes in human genomic DNA using denaturing gradient gel electrophoresis and a GC clamp.

doi:10.1016/0888-7543(90)90188-z

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Article

Comprehensive detection of single base changes in human genomic DNA using denaturing gradient gel electrophoresis and a GC clamp.

Genomics - 1 Aug 1990

Abrams E S, Murdaugh S E, Lerman L S

Abstract excerpt

We present a simple, efficient extension of denaturing gradient gel electrophoresis that allows the detection of nearly any sequence change in a defined fragment of DNA. The fragment can be obtained either by means of the polymerase chain reaction or by restriction digestion of genomic DNA. With...

Topics

Base Composition
Base Sequence
Cloning, Molecular
DNA
DNA Mutational Analysis
Deoxyribonucleases, Type II Site-Specific
Electrophoresis
Globins
Humans
Methylation
Molecular Sequence Data
Mutation
Plasmids
Polymerase Chain Reaction

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