Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Comprehensive detection of single base changes in human genomic DNA using denaturing gradient gel electrophoresis and a GC clamp.

Genomics - 1 Aug 1990

Abrams E S, Murdaugh S E, Lerman L S

Abstract excerpt

We present a simple, efficient extension of denaturing gradient gel electrophoresis that allows the detection of nearly any sequence change in a defined fragment of DNA. The fragment can be obtained either by means of the polymerase chain reaction or by restriction digestion of genomic DNA. With restriction fragments of genomic DNA, sequence information is not required, and covalent modifications in genomic DNA...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Base Sequence
Share to Cloning, Molecular
Share to DNA

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to DNA Mutational Analysis

Share to Molecular Sequence Data

Share to Mutation

Share to Plasmids

Share to Polymerase Chain Reaction

Source

Identifiers

PMID: 2387581
DOI: 10.1016/0888-7543(90)90188-z

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Comprehensive detection of single base changes in human genomic DNA using denaturing gradient gel electrophoresis and a GC clamp.PMID 2387581Read ↗

Mutation detection by denaturing gradient gel electrophoresis (DGGE)Semantic proximity Read ↗

High-resolution melting analysis (HRMA): more than just sequence variant screening.Semantic proximity Read ↗

PCR induction of a TaqI restriction site at any CpG dinucleotide using two mismatched primers (CpG-PCR).Semantic proximity Read ↗

Denaturing gradient gel method for mapping single base changes in human mitochondrial DNA.Semantic proximity Read ↗

Distinguishing different DNA heterozygotes by high-resolution melting.Semantic proximity Read ↗

Single nucleotide polymorphism screening with denaturing gradient gel electrophoresis.Semantic proximity Read ↗

Rapid detection of genetic mutations using the chemiluminescent hybridization protection assay (HPA): overview and comparison with other methods.Semantic proximity Read ↗

Simple, rapid detection of PCR heteroduplexes in DNA mutations and polymorphisms.Semantic proximity Read ↗

Unexpected behavior of H2Kb mutant DNAs in denaturing gradient gel electrophoresis.Semantic proximity Read ↗

Masking selected sequence variation by incorporating mismatches into melting analysis probes.Semantic proximity Read ↗

Melting curve analysis of SNPs (McSNP): a gel-free and inexpensive approach for SNP genotyping.Semantic proximity Read ↗

Enhanced annealing of mismatched oligonucleotides using a novel melting curve assay allows efficient in vitro discrimination and restriction of a single nucleotide polymorphism.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.