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Article

Detection of C677T mutation in methylenetetrahydrofolate reductase gene by denaturing high performance liquid chromatography.

Biomedical chromatography : BMC - 1 Nov 2004

Fang Nenghu, Lin Li, Ren Jicun, Wu Dan

Abstract excerpt

In this paper, we described an assay for the detection of the C677T mutation in the methylenetetrahydrofolate reductase (MTHFR) gene using denaturing high-performance liquid chromatography (DHPLC). The conditions for DHPLC analysis were systematically investigated based on a general HPLC instrument (Prostar VARIAN). A 225 bp DNA fragment covering the 677 site of MTHFR gene was amplified by PCR technology using...

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Source

Identifiers

PMID: 15386507
DOI: 10.1002/bmc.364

Genes

MTHFR

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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