Article
[Comparison of denaturing high performance liquid chromatography with direct sequencing in the detection of single nucleotide polymorphism].
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics - 1 Jun 2001
Shi J, Yang S, Jiang Z, Jiang H, Chen T, Chen Z, Huang W
Abstract excerpt
OBJECTIVE: Investigate the sensitivity and accuracy of the denaturing high performance liquid chromatography(DHPLC) technique for the detection of single nucleotide polymorphism(SNP). METHODS: Forty-one samples were detected by both DHPLC and direct sequencing. RESULTS: The comparison demonstrated that DHPLC detected all heterozygous sequences found by direct sequencing. No false-positive signals were seen in the...
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