Article
High selectivity detection of point mutation by DNA photochemical cross-linking.
Nucleic acids symposium series (2004) - 1 Jan 2006
Ogasawara Shinzi, Fujimoto Kenzo
Abstract excerpt
We describe the investigation of the photochemical ligation based DNA chip for enhanced discrimination of single nucleotide polymorphisms (SNPs). Results show that the reaction takes place over a period of hours on the target, reaching more than 50% stage in 10 min. Significantly, a single mismatch in the target sequence yielded extremely little ligated product, with a measured rate that was 10(3)-fold lower than...
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