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High-specificity single-tube multiplex genotyping using Ribo-PAP PCR, tag primers, alkali cleavage of RNA/DNA chimeras and MALDI-TOF MS.

Human mutation - 1 Jan 2013

Mauger Florence, Gelfand David H, Gupta Amar, Bodepudi Veeraiah, Will Stephen G, Bauer Keith, Myers Thomas W, Gut Ivo G

Abstract excerpt

Here, we describe a high-throughput, single-tube, allele-specific ribonucleotide analog pyrophosphorolysis-activated polymerization (ribo-PAP) PCR multiplex genotyping and resequencing method. An RNA/DNA chimeric PCR product is generated using genomic DNA as starting template, a panel of allele-selective 5'-tagged primers, a reverse primer, one nucleotide in the ribo-form (90-100%), the other nucleotides in the...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 23132774
DOI: 10.1002/humu.22227

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NOS1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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