Article
Suppression of insertions in the complex pdxJ operon of Escherichia coli K-12 by lon and other mutations.
Journal of bacteriology - 1 Mar 1992
Lam H M, Tancula E, Dempsey W B, Winkler M E
Abstract excerpt
Complementation analyses using minimal recombinant clones showed that all known pdx point mutations, which cause pyridoxine (vitamin B6) or pyridoxal auxotrophy, are located in the pdxA, pdxB, serC, pdxJ, and pdxH genes. Antibiotic enrichments for chromosomal transposon mutants that require pyridoxine (vitamin B6) or pyridoxal led to the isolation of insertions in pdxA, pdxB, and pdxH but not in pdxJ. This...
Topics
- ATP-Dependent Proteases
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Chromosome Mapping
- Cloning, Molecular
- Escherichia coli
- Escherichia coli Proteins
- Genetic Complementation Test
- Heat-Shock Proteins