Article
Identification and characterization of yeast mutants and the gene for a cruciform cutting endonuclease.
The EMBO journal - 1 Feb 1992
Kleff S, Kemper B, Sternglanz R
Abstract excerpt
An assay was developed that detected DNA cruciform cutting endonuclease activity in crude extracts of Saccharomyces cerevisiae. A collection of temperature-sensitive strains was screened using this assay, and a mutant lacking the activity was found. The mutation leading to the enzymatic defect wa...
Topics
- Amino Acid Sequence
- Base Sequence
- Chromosome Walking
- Chromosomes, Fungal
- Cloning, Molecular
- DNA, Fungal
- Endodeoxyribonucleases
- Genes, Fungal
- Genotype
- Holliday Junction Resolvases
- Molecular Sequence Data
- Mutation
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Sequence Homology, Nucleic Acid
- Temperature