Article
Molecular complementation of a collagen mutation in mammalian cells using yeast artificial chromosomes.
The EMBO journal - 1 Feb 1992
Strauss W M, Jaenisch R
Abstract excerpt
The cloning of large contiguous segments of mammalian DNA in Saccharomyces cerevisiae has become possible with the advent of Yeast Artificial Chromosomes (YACs). We are interested in extending the technique of genetic complementation analysis to the molecular level through the introduction of YACs into mammalian cells and the mammalian germline. We report the successful introduction of homogeneous DNA derived...
Topics
- Animals
- Chromosomes, Fungal
- Collagen
- Crosses, Genetic
- DNA
- DNA Probes
- Genetic Complementation Test
- Mice
- Muridae
- Mutation
- Restriction Mapping
- Saccharomyces cerevisiae