Article
Biochemical and biological activity of phosphorylated and non-phosphorylated ras p21 mutants.
Biochimica et biophysica acta - 11 Feb 1992
Chung H H, Kim R, Kim S H
Abstract excerpt
In contrast to all cellular ras oncogenes which carry a single activating mutation at codon 12, 13 or 61, all known retroviral ras oncogenes have two mutations at codons 12 and 59. To understand the role of the mutation at codon 59, we have constructed plasmids containing genes for Harvey ras: p21(Gly-12,Thr-59) and p21(Val-12,Thr-59). Escherichia coli expressed proteins and their respective phosphorylated (Pi)...
Topics
- Animals
- Base Sequence
- Chromatography, Gel
- Chromatography, Ion Exchange
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- GTP Phosphohydrolases
- Gene Expression
- Guanosine Triphosphate
- Kinetics
- Molecular Sequence Data