Article
Yeast and mammalian ras proteins have conserved biochemical properties.
Nature - 1 Jan 2000
Temeles G L, Gibbs J B, D'Alonzo J S, Sigal I S, Scolnick E M
Abstract excerpt
Mammalian ras oncogenes encode polypeptides of relative molecular mass (Mr) 21,000 (p21) which bind GTP and GDP. Oncogenic ras-encoded proteins differ from their normal homologues by an amino acid substitution for Gly 12, Ala 59 or Gln 61. Recently, we and others have observed that normal p21, encoded by the Ha-ras gene, has a GTP hydrolytic activity that is reduced by the oncogenic substitutions Val 12 or Thr...
Topics
- Fungal Proteins
- GTP Phosphohydrolases
- GTP-Binding Proteins
- Guanosine Triphosphate
- Kinetics
- Mutation
- Oncogenes
- Saccharomyces cerevisiae