Article
p21 with a phenylalanine 28----leucine mutation reacts normally with the GTPase activating protein GAP but nevertheless has transforming properties.
The Journal of biological chemistry - 15 Sept 1991
Reinstein J, Schlichting I, Frech M, Goody R S, Wittinghofer A
Abstract excerpt
The H-ras gene product p21H has been mutated at Phe-28, which makes a hydrophobic interaction with the guanine base of bound GDP/GTP. The mutation Phe-28----Leu drastically increases nucleotide dissociation rates without affecting association rates. This is due to a perturbed binding of base, alp...
Topics
- Animals
- Cell Transformation, Neoplastic
- Circular Dichroism
- GTP Phosphohydrolases
- GTPase-Activating Proteins
- Kinetics
- Leucine
- Magnetic Resonance Spectroscopy
- Mutation
- Phenylalanine
- Proteins
- Proto-Oncogene Proteins p21(ras)
- Temperature
- Tumor Cells, Cultured
- ras GTPase-Activating Proteins