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Article

Large-scale exploration of growth inhibition caused by overexpression of genomic fragments in Saccharomyces cerevisiae.

Genome biology - 1 Jan 2004

Boyer Jeanne, Badis Gwenaël, Fairhead Cécile, Talla Emmanuel, Hantraye Florence, Fabre Emmanuelle, Fischer Gilles, Hennequin Christophe, Koszul Romain, Lafontaine Ingrid, Ozier-Kalogeropoulos Odile, Ricchetti Miria, Richard Guy-Franck, Thierry Agnès, Dujon Bernard

Abstract excerpt

We have screened the genome of Saccharomyces cerevisiae for fragments that confer a growth-retardation phenotype when overexpressed in a multicopy plasmid with a tetracycline-regulatable (Tet-off) promoter. We selected 714 such fragments with a mean size of 700 base-pairs out of around 84,000 clones tested. These include 493 in-frame open reading frame fragments corresponding to 454 distinct genes (of which 91...

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Identifiers

PMID: 15345056
DOI: 10.1186/gb-2004-5-9-r72
PMCID: PMC522879

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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