Disruption and phenotypic analysis of six open reading frames from the left arm of Saccharomyces cerevisiae chromosome VII.

doi:10.1002/1097-0061(20000315)16:4<365::AID-YEA526>3.0.CO;2-2

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Article

Disruption and phenotypic analysis of six open reading frames from the left arm of Saccharomyces cerevisiae chromosome VII.

Yeast (Chichester, England) - 15 Mar 2000

Lillo J A, Andaluz E, Cotano C, Basco R, Cueva R, Correa J, Larriba G

Abstract excerpt

Six open reading frames (ORFs) from Saccharomyces cerevisiae chromosome VII were deleted using the kanMX4 module and the long-flanking homology-PCR replacement strategy in at least two different backgrounds. Among these ORFs, two of them (YGL100w and YGL094c) are now known genes which encode well-characterized proteins (Seh1p, a nuclear pore protein, and Pan2p, a component of Pab1p-stimulated poly(A)...

Topics

Chromosomes, Fungal
Fungal Proteins
Gene Deletion
Open Reading Frames
Phenotype
Plasmids
Restriction Mapping
Saccharomyces cerevisiae

Source

Open in PubMed

Identifiers

PMID: 10669874
DOI: 10.1002/1097-0061(20000315)16:4<365::AID-YEA526>3.0.CO;2-2

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