Article
Large-scale analysis of the yeast genome by transposon tagging and gene disruption.
Nature - 25 Nov 1999
Ross-Macdonald P, Coelho P S, Roemer T, Agarwal S, Kumar A, Jansen R, Cheung K H, Sheehan A, Symoniatis D, Umansky L, Heidtman M, Nelson F K, Iwasaki H, Hager K, Gerstein M, Miller P, Roeder G S, Snyder M
Abstract excerpt
Economical methods by which gene function may be analysed on a genomic scale are relatively scarce. To fill this need, we have developed a transposon-tagging strategy for the genome-wide analysis of disruption phenotypes, gene expression and protein localization, and have applied this method to the large-scale analysis of gene function in the budding yeast Saccharomyces cerevisiae. Here we present the largest...
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