Article
[Degradation of Cdc25A phosphatase in HeLa cells under normal and stress conditions].
Tsitologiia - 1 Jan 2004
Goloudina A R, Aksenov N D, Pospelov V A
Abstract excerpt
Cdc25A phosphatase regulates cell cycle progression by removing the inhibitory phosphates from cyclin-dependent kinases. Activity of Cdc25A depends on its phosphorylation status. During normal cell cycle progression and after DNA damage phosphorylation by Chk1 (or Chk2) triggers Cdc25A degradation via ubiquitin-proteasome pathway. In this study we investigate the role of various phosphorylation sites (Ser123,...
Topics
- Checkpoint Kinase 1
- DNA
- Enzyme Stability
- HeLa Cells
- Humans
- Mutagenesis, Site-Directed
- Mutation
- Phosphorylation
- Protein Kinases
- Serine
- Ultraviolet Rays
- cdc25 Phosphatases