Article
T cell receptor V beta gene usage in allograft-derived cell lines analyzed by a polymerase chain reaction technique.
Transplantation - 1 May 1992
Hall B L, Finn O J
Abstract excerpt
T cell receptor V beta gene usage in renal allograft-derived T cell lines was investigated using a semiquantitative, polymerase chain reaction (PCR)-based technique. A panel of V beta-specific primers was carefully designed to have uniform hybridization characteristics, enhancing comparability, and high specificity based on 3' mismatches between primers and nontarget V regions. PCR data were in concordance with...
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