The activation defect of a lambda cI positive control mutant.

Abstract excerpt

"Positive control" mutants of the cI protein of bacteriophage lambda (lambda cI) bind DNA but, unlike the wild-type protein, fail to activate transcription. According to the original interpretation of Ptashne and co-workers, these mutants bear amino acid substitutions that disrupt a stimulatory interaction between lambda cI bound at operator site O(R)2 and RNA polymerase bound at promoter P(RM), an idea supported...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Bacteriophage lambda
• Share to DNA-Binding Proteins
• Share to Mutation