Article
Single-tube method for nucleic acid extraction, amplification, purification, and sequencing.
Clinical chemistry - 1 Oct 2004
Margraf Rebecca L, Page Sam, Erali Maria, Wittwer Carl T
Abstract excerpt
BACKGROUND: The hepatitis C virus (HCV) genotype determines patient prognosis and duration of treatment, but sequencing of the gene is lengthy and labor-intensive. We used a commercially available nucleic acid extraction system to develop a single-tube extraction-to-sequencing (STETS) method for HCV genotyping. METHODS: HCV RNA was purified and amplified in tubes coated with a solid-phase matrix that irreversibly...
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