Article
Single-step nested polymerase chain reaction for detection of different genotypes of hepatitis C virus.
Journal of medical virology - 1 Feb 1995
Li J S, Tong S P, Vitvitski L, Trépo C
Abstract excerpt
Hepatitis C virus (HCV) exhibits considerable sequence variability and circulates in the blood at extremely low levels. Current methods for detecting HCV RNA are based mostly on nested polymerase chain reaction (PCR), in which part of the first amplification product is reamplified in the second t...
Topics
- Base Sequence
- DNA Primers
- Evaluation Studies as Topic
- France
- Genotype
- Hepacivirus
- Hepatitis C
- Humans
- Molecular Sequence Data
- Polymerase Chain Reaction
- RNA, Viral
- Sensitivity and Specificity