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Article

A simple, single-tube overlapping amplicon-targeted Illumina sequencing assay.

PloS one - 1 Jan 2023

Limberis Jason D, Nalyvayko Alina, Ernst Joel D, Metcalfe John Z

Abstract excerpt

Targeted amplicon sequencing to identify pathogens, resistance-conferring mutations, and strain types is an important tool in diagnosing and treating infections. However, due to the short read limitations of Illumina sequencing, many applications require the splitting of limited clinical samples between two reactions. Here, we outline hairpin Illumina single-tube sequencing PCR (hissPCR) which allows for the...

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PMID: 37708184
DOI: 10.1371/journal.pone.0288687
PMCID: PMC10501585

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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