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Analysis of solid-phase allele-specific primer extension characteristics on biochip in combination with modified primers and PicoGreen staining method.

Indian journal of biochemistry & biophysics - 1 Oct 2005

Qin Shengying, Liu Jianhua

Abstract excerpt

DNA microarray technology offers potential for future high-throughput variation genotyping. Allele-specific primer extension procedure on microarray has been considered as an efficient method for single nucleotide polymorphism(SNP) genotyping. However, the high cost of the fluorescent-labeled dNTP used for signal detection in this method limits its application. In the present study, we evaluated the...

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DNA
DNA Primers
Fluorescent Dyes
Oligonucleotide Array Sequence Analysis
Oligonucleotides

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Organic Chemicals

Sequence Analysis, DNA

Staining and Labeling

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PMID: 23923539

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