Article
Fluorescent-based single-strand conformation polymorphism/heteroduplex capillary electrophoretic mutation analysis of the P53 gene.
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry - 1 Jul 2004
Zhao Chunxia, Xu Guowang, Shi Xianzhe, Ma Jianmei, Zhang Yan, Lv Shen, Weng Qianfeng, Yang Qing
Abstract excerpt
Fluorescent-based single-strand conformation polymorphism (F-SSCP) analysis with capillary electrophoresis (CE) is the most common method for the detection of mutation because of its high sensitivity and resolution. In this study, we prepared an inexpensive linear polyacrylamide (LPA), and successfully applied it to CE-SSCP analysis and tandem CE-SSCP/heteroduplex analysis (HA) of the P53 gene on an ABI capillary...
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