Article
High-throughput single nucleotide polymorphism typing by fluorescent single-strand conformation polymorphism analysis with capillary electrophoresis.
Electrophoresis - 1 Mar 2004
Doi Kent, Doi Hitomi, Noiri Eisei, Nakao Akihide, Fujita Toshiro, Tokunaga Katsushi
Abstract excerpt
In this study, we performed high-throughput and precise single nucleotide polymorphism (SNP) typing by fluorescent capillary electrophoresis single-strand conformation polymorphism (CE-SSCP) analysis. A system composed of a multicapillary DNA analyzer, a newly developed sieving matrix, four different colors of fluorescent labels, and a multiplex polymerase chain reaction (PCR) enabled low-cost and highly reliable...
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