Functional analysis of SoxR residues affecting transduction of oxidative stress signals into gene expression.

Abstract excerpt

SoxR protein, a member of the MerR family of transcriptional activators, mediates a global oxidative stress response in Escherichia coli. Upon oxidation or nitrosylation of its [2Fe-2S] centers SoxR activates its target gene, soxS, by mediating a structural transition in the promoter DNA that stimulates initiation by RNA polymerase. We explored the molecular basis of this signal transduction by analyzing mutant...

Topics

• Amino Acid Sequence
• Bacterial Proteins
• Escherichia coli Proteins
• Gene Expression Regulation, Bacterial
• Models, Molecular
• Mutation
• Oxidation-Reduction
• Oxidative Stress
• Protein Conformation
• Sequence Alignment
• Signal Transduction
• Transcription Factors
• Transcription, Genetic