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Article

Efficient generation of transgenic mice with intact yeast artificial chromosomes by intracytoplasmic sperm injection.

Biology of reproduction - 1 Dec 2004

Moreira Pedro N, Giraldo Patricia, Cozar Patricia, Pozueta Julio, Jiménez Adela, Montoliu Lluís, Gutiérrez-Adán Alfonso

Abstract excerpt

The production of animals with large transgenes is an increasingly valuable tool in biotechnology and for genetic studies, including the characterization and manipulation of large genes and polygenic traits. In the present study, we describe an intracytoplasmic sperm injection (ICSI) method for the stable incorporation and phenotypic expression of large yeast artificial chromosomes (YAC) constructs of submegabase...

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PMID: 15286029
DOI: 10.1095/biolreprod.104.032904

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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