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Article

Locking of 3' ends of single-stranded DNA templates for improved Pyrosequencing performance.

BioTechniques - 1 Jul 2004

Utting Michael, Hampe Jochen, Platzer Matthias, Huse Klaus

Abstract excerpt

In Pyrosequencing, a DNA strand complementary to a single-stranded DNA (ssDNA) template is synthesized, whereby each incorporated nucleotide yields detectable light, and the light intensity is proportional to the incorporated nucleotides. Correct data interpretation (i.e., signal-to-noise ratio of light intensities) is hampered by artifacts due to the formation of secondary structures of single-stranded...

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PMID: 15283202
DOI: 10.2144/04371ST02

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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