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Article

Tail variation of the folding primer affects the SmartAmp2 process differently.

Biochemical and biophysical research communications - 12 Jun 2009

Kimura Yasumasa, Oguchi-Katayama Atsuko, Kawai Yuki, Naito Kana, Mitani Yasumasa, Morlighem Jean-Etienne, Hayashizaki Yoshihide, Lezhava Alexander

Abstract excerpt

Folding primer (FP), together with turn-back primer (TP) and boost primer (BP), is one of the major components of SmartAmp2, a rapid amplification-based method for SNP detection. FP has a unique design where the annealing region is combined with a tail that can fold back. FP tails can be classified as either "strong" or "weak", depending on the melting temperature and free energy of the hairpin structure. We...

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PMID: 19371726
DOI: 10.1016/j.bbrc.2009.04.033

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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