Article
Single-nucleotide polymorphisms and other mismatches reduce performance of quantitative PCR assays.
Clinical chemistry - 1 Oct 2013
Lefever Steve, Pattyn Filip, Hellemans Jan, Vandesompele Jo
Abstract excerpt
BACKGROUND: Genome-sequencing studies have led to an immense increase in the number of known single-nucleotide polymorphisms (SNPs). Designing primers that anneal to regions devoid of SNPs has therefore become challenging. We studied the impact of one or more mismatches in primer-annealing sites on different quantitative PCR (qPCR)-related parameters, such as quantitative cycle (Cq), amplification efficiency, and...
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