Article
SNP genotyping with FRET probes. Optimizing the resolution of heterozygotes.
Molecular and cellular probes - 1 Aug 2004
Martínez-García A, Sastre I, Tenorio R, Bullido M J
Abstract excerpt
Analysis of single nucleotide polymorphisms by PCR with fluorescence resonance energy transfer (FRET) probes often can produce a result where the melting peak corresponding to perfectly matched sequence (A allele) has a smaller area than the peak corresponding to the allele with a mismatch (B allele). This imbalance can make it difficult to distinguish heterozygous individuals from BB homozygotes. These results...
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