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Article

Directed evolution of an enantioselective epoxide hydrolase: uncovering the source of enantioselectivity at each evolutionary stage.

Journal of the American Chemical Society - 3 Jun 2009

Reetz Manfred T, Bocola Marco, Wang Li-Wen, Sanchis Joaquin, Cronin Annette, Arand Michael, Zou Jinyu, Archelas Alain, Bottalla Anne-Lise, Naworyta Agata, Mowbray Sherry L

Abstract excerpt

Directed evolution of enzymes as enantioselective catalysts in organic chemistry is an alternative to traditional asymmetric catalysis using chiral transition-metal complexes or organocatalysts, the different approaches often being complementary. Moreover, directed evolution studies allow us to learn more about how enzymes perform mechanistically. The present study concerns a previously evolved highly...

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Aspergillus niger
Directed Molecular Evolution
Epoxide Hydrolases
Kinetics
Mutation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Stereoisomerism

Substrate Specificity

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PMID: 19469578
DOI: 10.1021/ja809673d

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