Article
Structural studies on human glutathione S-transferase pi. Substitution mutations to determine amino acids necessary for binding glutathione.
The Journal of biological chemistry - 15 Sept 1992
Manoharan T H, Gulick A M, Puchalski R B, Servais A L, Fahl W E
Abstract excerpt
In order to identify amino acids involved in binding the co-substrate glutathione to the human glutathione S-transferase (GST) pi enzyme, we assembled three criteria to implicate amino acids whose role in binding and catalysis could be tested. Presence of a residue in the highly conserved exon 4 of the GST gene, positional conservation of a residue in 12 glutathione S-transferase amino acid sequences, and results...
Topics
- Amino Acid Sequence
- Amino Acids
- Base Sequence
- Blotting, Western
- DNA
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Glutathione
- Glutathione Transferase
- Humans
- Isoenzymes