Article
Structures of thermolabile mutants of human glutathione transferase P1-1.
Journal of molecular biology - 15 Sept 2000
Rossjohn J, McKinstry W J, Oakley A J, Parker M W, Stenberg G, Mannervik B, Dragani B, Cocco R, Aceto A
Abstract excerpt
An N-capping box motif (Ser/Thr-Xaa-Xaa-Asp) is strictly conserved at the beginning of helix alpha6 in the core of virtually all glutathione transferases (GST) and GST-related proteins. It has been demonstrated that this local motif is important in determining the alpha-helical propensity of the isolated alpha6-peptide and plays a crucial role in the folding and stability of GSTs. Its removal by site-directed...
Topics
- Amino Acid Substitution
- Crystallography, X-Ray
- Enzyme Stability
- Glutathione Transferase
- Humans
- Models, Molecular
- Mutation
- Protein Structure, Secondary