Article
Human glutathione transferase T2-2 discloses some evolutionary strategies for optimization of substrate binding to the active site of glutathione transferases.
The Journal of biological chemistry - 23 Feb 2001
Caccuri A M, Antonini G, Board P G, Flanagan J, Parker M W, Paolesse R, Turella P, Federici G, Lo Bello M, Ricci G
Abstract excerpt
Rapid kinetic, spectroscopic, and potentiometric studies have been performed on human Theta class glutathione transferase T2-2 to dissect the mechanism of interaction of this enzyme with its natural substrate GSH. Theta class glutathione transferases are considered to be older than Alpha, Pi, and Mu classes in the evolutionary pathway. As in the more recently evolved GSTs, the activation of GSH in the human Theta...
Topics
- Arginine
- Catalytic Domain
- Evolution, Molecular
- Flow Injection Analysis
- Glutathione
- Glutathione Transferase
- Humans
- Hydrogen-Ion Concentration
- Isoenzymes
- Kinetics