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Article

Insertional re-activation of a chloramphenicol acetyltransferase misfolding mutant protein.

Protein engineering - 1 Feb 1995

Robben J, Van der Schueren J, Verhasselt P, Aert R, Volckaert G

Abstract excerpt

The deletion of nine residues from the C-terminus of the bacterial chloramphenicol acetyltransferase (CAT) results in deposition of the mutant protein in cytoplasmic inclusion bodies and loss of chloramphenicol resistance in Escherichia coli. This folding defect is relieved by C-terminal fusion of the polypeptide with as few as two residues. Based on these observations, efficient positive selection for the...

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Source

Identifiers

PMID: 7630887
DOI: 10.1093/protein/8.2.159

Genes

CAT

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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