Article
Insertional re-activation of a chloramphenicol acetyltransferase misfolding mutant protein.
Protein engineering - 1 Feb 1995
Robben J, Van der Schueren J, Verhasselt P, Aert R, Volckaert G
Abstract excerpt
The deletion of nine residues from the C-terminus of the bacterial chloramphenicol acetyltransferase (CAT) results in deposition of the mutant protein in cytoplasmic inclusion bodies and loss of chloramphenicol resistance in Escherichia coli. This folding defect is relieved by C-terminal fusion o...
Topics
- Amino Acid Sequence
- Base Sequence
- Chloramphenicol O-Acetyltransferase
- Cloning, Molecular
- DNA
- DNA Restriction Enzymes
- Enzyme Activation
- Enzyme Reactivators
- Escherichia coli
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Polymerase Chain Reaction
- Protein Folding