Article
Study of heterogeneity of chloramphenicol acetyltransferase (CAT) genes in streptococci and enterococci by polymerase chain reaction: characterization of a new CAT determinant.
Antimicrobial agents and chemotherapy - 1 Dec 1993
Trieu-Cuot P, de Cespédès G, Bentorcha F, Delbos F, Gaspar E, Horaud T
Abstract excerpt
An assay based on the utilization of degenerate primers that enable enzymatic amplification of an internal fragment of cat genes known to be present in gram-positive cocci was developed to identify the genes encoding chloramphenicol resistance in streptococci and enterococci. The functionality of...
Topics
- Amino Acid Sequence
- Base Sequence
- Chloramphenicol O-Acetyltransferase
- DNA Primers
- Enterococcus faecium
- Gene Amplification
- Genes, Bacterial
- Genetic Variation
- Molecular Sequence Data
- Plasmids
- Polymerase Chain Reaction