Article
A simplified method for large scale quantification of transcriptional activity and its use in studies of steroids and steroid receptors.
Journal of receptor and signal transduction research - 1 Feb 2001
Zhang S, Lu J, Iyama K, Lo S C, Danielsen M
Abstract excerpt
Chloramphenicol acetyltransferase (CAT) is widely used as a reporter to determine the transcriptional specificity of promoters and for the quantification of transcriptional activity of transcription factors such as nuclear receptors. However, large-scale quantification of CAT activity in transfected mammalian cells is still heavily labor-intensive, time-consuming and expensive. Here, we describe a simplified...
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