Article
A dnaT mutant with phenotypes similar to those of a priA2::kan mutant in Escherichia coli K-12.
Genetics - 1 Jun 2004
McCool Jesse D, Ford Christopher C, Sandler Steven J
Abstract excerpt
The ability to repair damaged replication forks and restart them is important for cell survival. DnaT is essential for replication restart in vitro and yet no definite genetic analysis has been done in Escherichia coli K-12. To begin, dnaT822, an in-frame six-codon (87-92) deletion was constructed. DnaT822 mutants show colony size, cell morphology, inability to properly partition nucleoids, UV sensitivity, and...
Topics
- Base Sequence
- Codon
- DNA Primers
- DNA Repair
- DNA Replication
- DNA, Bacterial
- DNA-Binding Proteins
- Escherichia coli K12
- Escherichia coli Proteins
- Molecular Sequence Data
- Mutation
- Plasmids
- Polymerase Chain Reaction
- Sequence Deletion
