Article
ATPase-deficient mutants of the Escherichia coli DNA replication protein PriA are capable of catalyzing the assembly of active primosomes.
The Journal of biological chemistry - 5 Apr 1992
Zavitz K H, Marians K J
Abstract excerpt
The PriA replication protein of Escherichia coli (formerly replication factor Y or protein n') is multifunctional. It is a site-specific, single-stranded DNA-dependent ATPase (dATPase), a 3'----5' DNA helicase, and guides the ordered assembly of the primosome, a mobile, multiprotein DNA replicati...
Topics
- Adenosine Triphosphatases
- Base Sequence
- Catalysis
- DNA Helicases
- DNA Replication
- DNA, Bacterial
- DNA-Binding Proteins
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Plasmids
- Replication Protein A